Wanunu Lab 🇺🇸 @WanunuLab
Single-molecule biophysics and nanotechnology laboratory nuweb9.neu.edu/wanunu/ Northeastern University Joined September 2014-
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Be there! Superb nanopore conference in Thailand smps2026.nstda.or.th Abstract submission deadline is June 30, 2026
Earlier this week I posted an example of a fake western blot provided by ThermoFisher to demonstrate the validity of a p53 antibody. I considered it an amusing curiosity. In fact ThermoFisher has systematically manipulated antibody validation data. Short Thread... 🧵
The generated code can be used offline in future analysis. Want to plot, compare, visualize? Try NanoCortex! biorxiv.org/content/10.648…
Introducing NanoCortex, a chat-based nanopore agent for processing nanopore data. We hope it will be useful for people in the nanopore field with little to no coding skills. Disclaimer: use of the agent required API tokens (there is an associated cost). However...
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Surprised to discover that Thermo Fisher appears to show a fake western blot for the validation of one of their p53 antibodies. I've added a diagram to show the very similar bands. This does not appear to be one of the "published figures", but their own internal data.
What does pseudouridine (ψ) actually do to protein production? It’s been studied for years — but its impact on translation has remained surprisingly unclear. We were excited to tackle this directly in our latest NAR paper: tinyurl.com/psitranslation
This is why international scientific exchange is so important. We must protect and encourage collaboration and mobility.
What if AI could invent enzymes that nature hasn’t seen? 👩🔬🧑🔬 Introducing 🪩 DISCO: Diffusion for Sequence-structure CO-design 14 rounds of directed evolution and over a year of wet lab work. That's what it took to engineer an enzyme for selective C(sp³)–H insertion, one of the
Help build a sub $500 DNA sequencer 🧬 Seeking an intern experienced in 4-dye SBS chemistry + surface DNA amplification. Real system, real chemistry, open source. ~200M bases/run, <$50 per run. Location: yacht in the Mediterranean & Aegean. Must be hands-on and able to build + troubleshoot. DM @GangstaQuack
@slavov_n How many grams of iron would one need to uptake?
This was a fruitful collaboration between our group and the @ShemaEfrat lab at @WeizmannScience and Sean O'Leary at @UCRiverside.
Incubating primed NucBells with polymerase and immobilizing them on the @PacBio RSII platform (I know, outdated, but versatile!) allows histone marks to be detected using fluorescent immunoaffinity reagents. Then, following a wash step, the DNA is sequenced.
Pleased to share our latest manuscript that introduces NucSeq, a SMRT-sequencing based single-molecule approach to interrogate histone PTMs and sequence the wrapped nucleosomes around these histones. pubs.acs.org/doi/10.1021/ac…
I recently learned that RNase A enzymes can survive autoclaving, or high-pressure heating, at 121°C. This is strange. But then I learned there are entire organisms that not only survive autoclaving, but actually grow and divide at 121°C??? There was a 2003 paper, for example, where these two scientists took a submarine down to a hydrothermal vent in the Pacific ocean, scooped up some dirt, and kept everything in an airtight tube. This tube had an organism in it. The organism had features "typical of Archaea." They put this organism into an autoclave (held at 121°C) for a full 24 hours. When they took it out of the autoclave, the cell population had doubled. Thus, the organism was called "Strain 121." The 2003 paper ends with an enticing statement: "The factors that permit strain 121 to grow at such high temperatures are unknown. It is generally assumed that the upper temperature limit for life is related to the instability of key molecules essential for life, but which molecules are most important in defining the upper temperature limit have not been defined. However, strain 121 offers the possibility to do this work." I read this and got excited. I began searching for follow-up studies on Strain 121. But I was quickly disappointed. This organism has its own Wikipedia page, but every single reference is from 2003 or 2004. Its name was later changed to Geogemma barossii, so I searched for that, too. But all I could find were random news stories about this "heat-loving microbe," all of which linked back to the original 2003 paper. I'm extremely confused by this. Why is nobody studying this microbe? It doesn't even have a published genome sequence. Where is the intellectual center for hyperthermophile research?
Excited to take part in this effort to bring protein sequencing to reality! darpa.mil/news/2026/prot… @NortheasternCOS @NUBioE1 @Northeastern
DARPA Biological Technologies Office: Protean (Feb 20) sam.gov/workspace/cont…
I started a company! Luminos. We made a complete neuro-electronic interface: stimulate and record from any set of neurons in any pattern of space and time, over a 6 mm field of view. Also useful for optical pooled screens and target discovery. luminosmicro.com
Today’s American History Lesson: Puerto Rico has been part of the U.S. since 1898, and Puerto Ricans have been U.S. citizens since 1917 🇵🇷 🇺🇸 We’ve cheered many non-Americans at the #SuperBowl. Bad Bunny isn’t one of them. 🏈 🎤
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Prof. Nikolai Slavov @slavov_n
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578 Followers 156 Following Soft matter physics and rheology, science-based AI solutions and computational tool development at Mechanical engineering @Northeastern
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