Ning Zhao @NingInScience
Asst. Prof. @ CU-Anschutz | gene regulation and protein folding | single-particle tracking | live-cell imaging | she/her medschool.cuanschutz.edu/biochemistry/p… Colorado, USA Joined November 2018-
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@JMTali Beautiful image! Congrats on the exciting finding!
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@Steve_Bonilla @pewtrusts Congrats, Steve!
@Guiping_W @CRUK_CI Congrats! This's so exciting.
@angalus_d @cdasno Thanks, Sulagna! Let me know if you need the plasmids or sequence files.
We have developed a cysteine-free, highly thermostable tagging system, UTag, that enables single-mRNA translation tracking in live cells. You may wonder how different tagging systems affect translation kinetics—we addressed this by performing a systematic comparison.
We benchmarked UTag against SunTag and ALFA-tag for single-mRNA translation tracking and observed comparable translation spot intensity and signal-to-noise ratios. Importantly, translation kinetics were also comparable across systems, as determined by both ACF and harringtonine.
To further enhance its robust folding in intracellular environments, we engineered a cysteine-free variant with improved thermostable (Tm ~80 °C) that maintains the high UTag binding affinity. Excitingly, both intrabodies enable single-mRNA translation tracking in live cells.
New preprint from our lab, led by Luis Aguilera! We have developed an open-source software, "MicroLive," to track single molecules in live cells. This one-step platform enables researchers without coding experience to perform single-molecule studies in a user-friendly interface!
MicroLive: An Image Processing Toolkit for Quantifying Live-cell Single-Molecule Microscopy biorxiv.org/content/10.110… #biorxiv_biophys
@FrydmanLab @scafrancesco1 @DeuerlingLab We are going to discuss this paper in our journal club today. 😀
Imaging single endogenous RNA in live cells comes true!
Single-molecule live-cell RNA imaging with CRISPR–Csm go.nature.com/3EJPKsM
@EricTopol @DoudnaLab @NatureBiotech The RNA signal is so good. Beautiful work!
Ning Zhao retweeted
Visualizing RNA in live cells using CRISPR @DoudnaLab @NatureBiotech nature.com/articles/s4158…
Ning Zhao retweeted
IgGM: A Generative Model for Functional Antibody and Nanobody Design 1/ IgGM is a novel generative model designed to facilitate the de novo design of antibodies and nanobodies with functional specificity, focusing on their ability to bind specific antigens. This model uniquely generates both the sequences and structures of antibodies in a single step. 2/ The model consists of three main components: a pre-trained language model that extracts sequence features, a feature learning module to identify relevant features, and a prediction module to generate antibody sequences and predict the antibody-antigen complex structure. 3/ IgGM addresses a major challenge in antibody design by generating novel antibody sequences and their structures without the need for existing experimental antibody-antigen complex structures, making it suitable for scenarios involving new antigens. 4/ The model is capable of designing antibody CDR regions, which are crucial for antigen binding, and can also predict and design entire antibody structures, a significant advancement over existing methods that often require pre-existing structures or templates. 5/ IgGM excels in both antibody and nanobody design tasks, achieving superior performance in multiple design scenarios, such as generating antibodies with high specificity and binding affinity to antigens, outperforming previous methods in terms of sequence and structure fidelity. 6/ Its ability to design antibodies and nanobodies for specific epitopes, including the design of multiple CDR regions simultaneously, provides a versatile solution for therapeutic and diagnostic applications. 7/ Experimental results demonstrate IgGM's impressive accuracy, including higher success rates in docking performance and lower RMSD values compared to existing methods, showcasing its potential in the rapidly growing field of AI-driven drug design. 💻Code: github.com/TencentAI4S/Ig… 📜Paper: biorxiv.org/content/10.110… #AntibodyDesign #NanobodyDesign #GenerativeModels #MachineLearning #AIinHealthcare #DrugDiscovery #Bioinformatics #ComputationalBiology #DeepLearning #AIforMedicine #ProteinDesign
@jjanetzko @FaboPolanco We have interacted before. Small world, John!
@FaboPolanco Good luck with your next step, Fabian!
Ning Zhao retweeted
Emergency town hall at #BPS2025 Impact of U.S. policies on biophysics research and what you can do about it. Tuesday, February 18 1:30 pm - 3:30 pm Room 411
Ning Zhao retweeted
Making intrabodies from antibodies just got easier! Learn how we made 𝟭𝟵 intrabodies to bind and light up peptides and histone modifications in live cells. And thanks to Academia, all sequences are freely available. (video credit: Yuko Sato @YukoSatoT2) biorxiv.org/content/10.110…
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