David Dulin @Dulin_Lab
Associate Prof. @VUamsterdam. #SARSCoV2 replisome, innate immune response, and nucleic acid processing motors at the single molecule level. daviddulinlab.com Amsterdam, The Netherlands Joined October 2016-
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@CameronLabUNC Many congrats, so well deserved!!
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@CameronLabUNC Our pleasure! Thank you and Jamie for coming over and make these holidays truly special! 🤗❤️
David Dulin retweeted
Our study with @xiangylab is out in JVi and was selected as an Editor’s Pick. This study addresses a longstanding question in coronavirus biology: How much proofreading does SARS-CoV-2 actually need? Coronaviruses rely on nsp14 ExoN to preserve their unusually large RNA genomes, yet catalytic-site mutations were thought to be lethal in SARS-CoV-2. We combined a new continuous fluorescence assay for ExoN activity with an optimized single-bacmid reverse-genetics system to connect enzyme activity directly to viral viability and evolution. Mutations produced a graded series: K9A retained robust activity and supported efficient recovery; catalytic D90A/E92A substitutions sharply impaired ExoN yet yielded a viable, slow-growing virus; and the more disruptive K9E substitution prevented recovery. Crucially, the D90A/E92A virus maintained its engineered substitutions through serial passage while accumulating genome-wide variation,more than 400 SNPs by passage 14 versus fewer than 40 for wild type, establishing a stable mutator phenotype. The work replaces a simple “essential or dispensable” view of proofreading with a quantitative threshold model: SARS-CoV-2 can survive substantial loss of ExoN activity, but not below a minimum functional level. That insight provides a framework for understanding coronavirus genome stability, fitness, evolution, attenuation, and vulnerability to fidelity-disrupting antivirals. doi.org/10.1128/jvi.00…
David Dulin retweeted
Another step towards assembly of the coronavirus replicase. In this latest report, @Dulin_Lab and collaborators demonstrate a role of the nsp13 helicase in helping the nsp12 polymerase and accessory factors address secondary structures in the RNA template. cell.com/cell-reports/f…
David Dulin retweeted
New work from @RKirchdoerfer lab in collaboration with Jamie Arnold and @Dulin_Lab reveal the potent, inhibitory activity of arabinoside incorporation on elongation by both coronavirus and enterovirus RNA polymerases. The major problem with utilization of this class of nucleoside is the poor conversion to the triphosphate in the cell. We are now working on strategies to address that issue. jbc.org/article/S0021-…
David Dulin retweeted
Interested in our recent norovirus NS3 work? 👇 Join us to dig deeper! I'm recruiting a PhD candidate to investigate NS3's role in viral replication and assembly using #cryoEM, #cryoET, and other complementary techniques. 🔗 tinyurl.com/4f3t3aae Please share! 🙏
Latest preprint from the lab, many years in the making! By combining #cryoEM with #AlphaFold3 modelling, we propose that NS3 forms a transmembrane RNA translocase. This could have big implications for our understanding of norovirus replication (🧵) 🔗biorxiv.org/content/10.110…
@CameronLabUNC Great food and great company, what else could I ask for? 😊
David Dulin retweeted
The IncaRNAto lab is seeking a motivated #PhD candidate, interested in studying #RNA structural ensemble dynamics in living cells. Here's the link to the vacancy: rug.nl/about-ug/work-…. Please share!
Thanks for having us on this nice work, @DanielHurdiss! ☺️
@MRHerodDR Does our hex-NS3 construct bind RNA? 🧬 Yes! Single-molecule magnetic tweezers experiments with the @Dulin_Lab indicate binding to ssRNA and dsRNA, with compaction of the latter. No ATP-driven helicase activity detected (thoughts on that in the paper).
@FMorenoHerrero @NAR_Open @CNB_CSIC Nice work!
@b_subhas @HaldarShubhasis @snbkol @DBTIndia Congrats Subhas, super well deserved!
David Dulin retweeted
A new preprint. We reveal the response of the enterovirus polymerase to a break in the template its copying. Different from homologous recombination. This attribute will facilitate attachment of adapters to RNA for applications like nanopore sequencing. biorxiv.org/content/10.110…
@Speck_Lab @morvorenlowen @ma89rta Congrats Christian!
@Farehlab @ScienceAdvances @C_Shembrey Congrats Mo!!
@OlivierDuss @ERC_Research @embl Congrats!
@tinekelenstra Congrats Tineke, well deserved!
David Dulin retweeted
Last call before its gone! Apply by Nov 25th...
We have 2 postdoc openings for an imaging biophysist and a data scientist at @KavliOxford. Intrigued by super-res microscopes🔬, single molecules, RNA, condensates, AI and bacteria 🦠? Read on! #ScienceJobs #Postdoc #bacteria #singlemolecule Pls RT.
David Dulin retweeted
Official pictures and recap from NWO Biophysics 2024 are now available! Thanks to everyone who contributed to the success of this event and hopefully until next year at NWO Biophysics 2024! View the pictures and recap here: nwobiophysics.nl/imagery. Vertaling weergeven
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